4-ACO-DMT, O-Acetylpsilocin is a synthetically created psychoactive substance that has been suggested by David Nichols to be a potentially useful alternative to psilocybin for pharmacological studies, as they are both believed to be prodrugs of psilocin.

Given that psilacetin is likewise active when injected, it is believed that 4-AcO-DMT/psilacetin is deacetylated into psilocin in the body during first pass metabolism and subsequent passages through the liver. This is based on reports that most users are unable to discriminate between these two substances when taken, to the point where they are frequently thought to have similar subjective effects. It has not been scientifically confirmed, though.Nonetheless, some people assert that psilocin’s acetylated and non-acetylated versions have subjectively different effects.

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